The following operations are done:
1. threshold the volume, using a user-preset threshold; 2. apply
a 33
1 erosion followed by a
3
3
1 dilation (i.e., these two operations
constitute a 3
3
1 opening) to smooth the
thresholded result [25,19]; 3. save the largest connected object
> 25 voxels in size.
threshold = 0